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The dried mannitol-free was injected to UPLC installed with a TSKgel UP-SW3000 column (Tosoh Bioscience LLC, King of Prussia, PA, USA). The mobile phase was composed of 0.1 M potassium dihydrogen phosphate buffer and 0.1 M disodium monohydrogen phosphate buffer (1:1, v:v) and the flow rate was set to 0.2 mL/min. The column temperature was 30°C and the sample temperature was …

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