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Images were acquired at 20× using Aperio AT2 (Leica, Germany). Mosaic images were acquired of the whole brain and sections of interest were identified. The % infarct area was evaluated in brain sections at bregma level + 1.0, 0.0 and −1.5 mm

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Aperio Slide Scanning Core Our instrument is a Leica Biosystems AT2, acquired in 2013. Technical specifications for this instrument can be found here. The AT2 can scan either 1x3 inch or 2x3 inch microscope slides. It has a rotating auto-feed drum which can accommodate up to 400 slides at a time. It has a single 20x objective lens; 40x magnification is achieved through use of a 2x "doubler

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The ImageJ SURF 1 and OpenCV software libraries registered each representative to an \(800\times 800\) px image patch taken from the high-resolution Aperio …

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Analyzing Immunohistochemically Stained Whole-Slide Images 3 In order to circumvent these restrictions, we propose to learn the deconvolu-tion in a conventional machine-learning setting, based on a very limited amount

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